Synthesis of double-clickable functionalised graphene oxide for biological applications.

نویسندگان

  • Kuo-Ching Mei
  • Noelia Rubio
  • Pedro M Costa
  • Houmam Kafa
  • Vincenzo Abbate
  • Frederic Festy
  • Sukhvinder S Bansal
  • Robert C Hider
  • Khuloud T Al-Jamal
چکیده

Azide- and alkyne-double functionalised graphene oxide (Click(2) GO) was synthesised and characterised with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA) and Raman spectroscopy. Fourteen-percentage increase in azide content was found, after pre-treatment of GO with meta-chloroperoxybenzoic acid (mCPBA), determined with elemental analysis. No effect on A549 cell viability was found, up to 100 μg mL(-1) and 72 h of incubation, determined with the modified lactate dehydrogenase (mLDH) assay. Two sequential copper(i) catalysed azide-alkyne cycloaddition (CuAAC) reactions were performed to conjugate the propargyl-modified blood-brain barrier targeting peptide Angiopep-2, and a bis-azide polyethylene glycol (MW = 3500), to the Click(2) GO. The final conjugate was characterised with ATR-FTIR and TGA.

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عنوان ژورنال:
  • Chemical communications

دوره 51 81  شماره 

صفحات  -

تاریخ انتشار 2015